Composite
XK

Part:BBa_K4324002:Design

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-10-02)


lacPO-RBS-XK-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1007
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A nucleotide sequence was found from the protein sequence, for expression in E. coli.

Source

This original protein sequence was taken from the xylB gene of Escherichia coli (strain K12), which can be found on the Uniprot database.

Alternatively, the protein sequence is pasted below:

MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQDPEQWWQATDRAMKALG DQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCAQECTLLEARVPQSRVITG NLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRMTGEFASDMSDAAGTMWLDVA KRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAKAWGMATVPVVAGGGDNAAGAVG VGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHSFCHALPQRWHLMSVMLSAASCLDW AAKLTGLSNVPALIAAAQQADESAEPVWFLPYLSGERTPHNNPQAKGVFFGLTHQHGPNE LARAVLEGVGYALADGMDVVHACGIKPQSVTLIGGGARSEYWRQMLADISGQQLDYRTGG DVGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAYQPRRETFRRLYQQLL
PLMA

References

1. https://www.uniprot.org/uniprotkb/P09099/entry